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However, TMEM55B lacks sequence similarity to other lipid phosphatases, and in vitro activity as a lipid phosphatase could not be validated in a later study 33

and 510 min incubation to overcome the limitation of auto-oxidation similar to the report of Paroni et al

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The 28-day window assumes USP-spec composition at day zero

Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay

Furthermore, CERT1 depletion was abrogated dose-dependently by preincubation with the CERT1 ligand 366 (Fig