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The powder was resuspended in 200 l of polysome buffer (50 mM Tris-HCl [pH 7.5], 100 mM KCl, 12 mM MgCl 2 , 1% Igepal CA-630, 1 mM dithiothreitol, 200 U/mL RnaseOUT, 1 mg/mL heparin sodium salt, 100 g/mL cycloheximide plus EDTA-free protease inhibitor cocktail in DEPC water), mixed by pipetting, and centrifuged at 15,000 RPM at 4 C

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