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One ml of the bacterial suspensions was washed twice in Phosphate Buffered Saline (PBS, pH 7.2) at 5000 g, 4C, for 10 min and the optical density assessed by spectrophotometry at 600 nm

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Addingly, another study not only showed increased iron concentration in SN pars compacta of early-stage PD patients compared to controls, but in those with late-stage PD apart from affecting SN pars compacta, other areas such as red nucleus and globus pallidus became involved suggesting a regionally progressive accumulation that is consistent with the stage of the disease (Guan et al., 2017)

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