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The membrane was washed three times in PBS and incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody (Amersham International) for 1 h

Rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) complex 1, exhibits cardioprotective effects in ferroptotic cells, where mTOR overexpression protects against iron-overload-induced cell death, a process exacerbated by mTOR deficiency (67, 68)

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