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After de-paraffin Cartilage specimens and Cells (in vitro) were washed in PBS and for deactivating endogenous peroxidase action by the use of 3% Hydrogen peroxide (H 2 O 2 ) for 20 min at room temperature with shaking

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When given to children aged 8-17 years who were simultaneously receiving stable psychiatric treatment (drug and therapy), NAC (up to 2.7 g/day for 12 weeks) significantly reduced OCD symptoms starting in week 8

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