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Long-Term Substitutions Patients seeking the antioxidant or anti-inflammatory benefits of GTE during isotretinoin therapy can consider: Dietary brewed green tea at 1 to 3 cups per day with meals (not supplements) Topical EGCG formulations, which bypass hepatic first-pass entirely and have shown benefit in acne management in the JID 2012 RCT [11] Reassessing GTE supplementation after isotretinoin completion and a minimum 4-week washout period Drug Interactions With Isotretinoin Beyond GTE: Context Matters GTE is not the only supplement concern during isotretinoin therapy

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Serum was separated by centrifugation at 800 g for 10 min at room temperature

To achieve this, we compared data from G49-treated mice in the presence of either or both antagonists to their respective control groups (antagonists in the absence of G49)