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The mixture (100 L) was added to the slides precoated with 1.5% normal-melting-point agarose to retain the agarose cell suspension

Nicotinamide N-methyltransferase (NNMT) is an enzyme that methylates nicotinamide using SAM as a methyl donor

Gently massage into the scalp and leave on overnight

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Refrigerate at roughly 28 C, protect from light, do not freeze, and swirl rather than shake

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