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doi: 10.1016/j.cell.2011.07.033 18 LiMChengHTianDYangLDuXPanYet al

Samples were allowed to cool, and 1.75 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 2 ml

TMT liquid chromatography-mass-spectrometry (LC-MS) analysis Samples were analyzed by liquid chromatography-tandem mass spectrometry using an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific) coupled to an UltiMate 3000 Series Rapid Separation LC system and autosampler (Thermo Scientific Dionex)

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Frequency: Once per day (subcutaneous, preferably before sleep) [3]

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