Nishimura M
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Protein was eluted with 10 mM glutathione in 50 mM Tris, pH 8.0, 5% glycerol, and examined for purity by SDS-PAGE and Coomassie staining
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Total IgG, IgG1, IgG2 and IgA antibodies coupled with IFN- and IL-2 expression in peripheral blood mononuclear cells, splenocytes and lymph node cells, but no IgG3 and IgG4 were elicited in either group, indicative of a mixed/balanced Th1/Th17 and Th2 response ( Human correlate studies revealed Sm-p80 reactivity with immunoglobulin G in human serum samples from schistosome-infected individuals