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Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37C

doi:10.1016/S0022-3565(25)22106-1

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In ARF, antibodies against the M antigen found in the streptococcal cell wall cross react with cardiac myosin causing carditis

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Khavinson VK, Bondarev IE, Butyugov AA