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Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes

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The pen form can be described as a dosing applicator designed for research applications, enabling standardised reagent portioning within RUO laboratory procedures

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Available at: Accessed 7/21/25