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Briefly, in order to qualitatively test whether FSP1 generates H 2 O 2 , reaction mixtures containing 500 M NAD(P)H, FSP1 (2 M cFSP1 or hFSP1, or no protein) and CoQ 1 (0 or 200 M) in buffer C (50 mM Tris-HCl pH 8.0, 250 mM NaCl) were incubated at room temperature for 20 min

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This biosensor was targeted to specific subcellular regions, including the nucleoli, stress granules, p-bodies, nuclear paraspeckles and Cajal bodies, along with their respective surrounding environments, i.e., the cytosol and nucleoplasm

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Citation: Prkacin, I., Separovic, J., & Sikiric, P