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After undergoing two washes with PBS, the cells were serum-deprived for 1 h in KrebsRinger bicarbonate HEPES (KRBH) buffer including 1% bovine serum albumin (BSA), followed by KRBH buffer containing 1% BSA and 11.1 mM glucose for 0.5 h

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These findings emphasize that targeted antagonism of NMDA receptor signalling lowers body weight independently of MC4R, indicating that this preclinical drug candidate might not only be effective in treating polygenic obesity but also holds potential as an effective weight loss strategy for patients with loss-of-function mutations in MC4R , the most common form of monogenic obesity 32

However, patients may experience some initial side effects as their bodies adjust to the medication