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Colonic tissue samples were collected from lesion sites (2 6 mm), fixed with 4% paraformaldehyde, embedded in paraffin, sliced (5 m thick), and stained with H&E stains as per the manufacturers protocol [24]

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To prepare the long single-stranded editing donor DNA (lssDNA), the 1.5 kb insert was amplified from slc25a39-flox-E2-Puc57 and processed to lssDNA using exonucleolytic hydrolysis of its 5-phosphorylated DNA strand as described previously 44