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Prcautions demploi

Plates were kept at 37 C in a humidified incubator in the presence of 5% CO 2

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For researchers entering the field of regenerative peptides, GHK-Cu is the logical starting point

The HBSS was removed, and the cells washed four times with ice cold HBSS (pH 7.4), before fresh growth medium without FCS and supplemented with MEM non-essential amino acids (ThermoFisher) was added to one of the plates and incubated ON before samples were collected (recycling fraction)

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