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Session four

Immunoassay Tear fluid was extracted from half of the remaining strip by agitating small cut pieces of these strips in 100 L PBS, Tween20 0.05% and cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) at 4 C for 1.5 h 28

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Serum l-carnitine showed significant positive correlation with IIEF-5 scores ( r = 0.873, P = 001), serum 25(OH)D ( r = 0.796, P = 0.001) and significant negative correlation with the age ( r = -0.515, P = 0.001)