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Five micrograms of the peptide were loaded onto a C18-reversed-phase column in buffer A (2% acetonitrile and 0.1% formic acid) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid) at a flow rate of 250 nL min 1 controlled by IntelliFlow technology over 120 min

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[DOI] [PMC free article] [PubMed] [Google Scholar] 4.Whytock KL, Carnero EA, Vega RB, Tillner J, Bock C, Chivukula K, Yi F, Meyer C, Smith SR, Sparks LM