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Importantly, several studies have reported acceptable pregnancy rates using cooled or cryopreserved epididymal sperm in horses, supporting its relevance for assisted reproduction and genetic conservation (42)

Stand on a step with heels hanging off the edge Rise up on toes using both legs Shift weight to affected leg only Slowly lower heel below step level over 3 to 5 seconds Return to starting position using both legs Perform 15 repetitions, 3 sets, twice daily Expect some discomfort during exercise

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Rapid dehydration was performed using graded ethanol (95% and 100%, 30 s each), followed by xylene clearance (twice, 5 min each)

Although, depending on the absolute genus abundance, Methanobrevibacter could be directly related to increased methane emissions, it could also present opportunities for biogas production, following the re-use of excreta in anaerobic digesters [66]

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