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Redox regulation of immunometabolism

Twenty-four hours later, medium was collected and stored at 20 C for further analysis, and cells were harvested, rinsed twice with ice-cold PBS, and lysed with RIPA buffer (140 mM NaCl, 20 mM Tris, pH=7.4, 10% glycerol, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulfonyl fluoride and leupeptin at 20 g ml 1 )