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Neuroreport 19:589593 [DOI] [PubMed] [Google Scholar] Rosenbaum DM, Zhang C, Lyons JA, Holl R, Aragao D, Arlow DH, Rasmussen SG, Choi HJ, Devree BT, Sunahara RK, et al

But it is short enough that each aliquot only gets punctured five to seven times and spends less than a week in the fluctuating environment of an active refrigerator

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These numbers are general guidelines