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C57BL/6J wild-type (WT) mice were purchased from Jackson Laboratory (Bar Harbor, ME)

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After 24 h, the supernatant was removed, and biofilms stained with LIVE/DEAD BacLight stain (Invitrogen) for 10 min (as performed in the Biofilm Formation assay) prior to CLSM imaging using a Leica TCS SP5 CLSM