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LaccID activity is specific to the mammalian cell surface In mammalian cells, LacAnc100 is active at the cell surface but fusions targeted to the cytosol, mitochondria or endoplasmic reticulum (ER) lumen are inactive (Supplementary Fig

Measurements were taken in a BioTek synergy H1 microplate reader

Absorbing vitamin B12 requires the secretion from the Intrinsic factor which is a glycoprotein produced by the parietal cells in the stomach

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