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Prior to transfection, 200 ng of siRNA and 3 l Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) were mixed and incubated at room temperature for 15 min in serum-free media, then added dropwise on top of the cells

Both are administered intranasally

doi: 10.1038/s41467-020-19249-z

In addition, red drum also grew faster when fed diets supplemented with 2% creatine or 1% guanidinoacetate [41], when compared with fish without creatine or guanidinoacetate supplementation

Rotterdam: Erasmus MC Press.

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