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TNF was added to the culture media at 17 mg/ml, 36 h prior to assaying lipolysis

C-peptide levels are measured instead of insulin levels because C-peptide can assess a person's own insulin secretion even if they receive insulin injections, and because the liver metabolizes a large and variable amount of insulin secreted into the portal vein but does not metabolise C-peptide, meaning blood C-peptide may be a better measure of portal insulin secretion than insulin itself

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It may also increase your risk of becoming dehydrated, which could result in kidney damage in rare cases