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The mean duration of diabetes was 18 years and the mean HbA1c at baseline was 7.8%

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If you need regular injections of vitamin B12, cyanocobalamin would need to be given once a month, whereas hydroxocobalamin can be given every 3 months

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Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 l volume containing 7.5 l eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 l 5 RT buffer (Promega), 30 U RNasin RNase inhibitor (Promega), and 0.75 g random hexadeoxynucleotides (Roche) in order to retrotranscribe the whole HCV genome preventing any bias during the reaction