They were transiently transfected with truncated GLP-2 receptors attached to LgBiT and G-SmBiT (or G-HiBiT) in a ratio of 1:1 using Lipofectamine 2000 transfection reagent (Invitrogen) and incubated for 24 h
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Available from: [10] Enberg N, Alho H, Loimaranta V, Lenander-Lumikari M
This matters because weight-loss treatment is increasingly personalized based on metabolic biomarkers and genetic predispositions rather than standardized protocols
In our experience, a diet focused on high-quality proteins, vegetables, and slow-burn carbohydrates tends to be a healthy and sustainable diet for most patients