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Furthermore, the BDNF-TLR4 axis is compromised during ALI through a self-amplifying inflammatory loop: TLR4 upregulation enhances sensitivity to inflammatory stimuli 28 , while NF-B activation by the ensuing cytokine storm may further suppresses BDNF transcription 29

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Second staining Subsequently, sections previously stained for S100, MAOB, and ABAT were incubated with guinea pig anti-GABA (1:100, Millipore ab175) and chicken anti-GFAP (1:200, Abcam ab4647) antibodies for 24 h

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Proper experimental structure and documentation are critical when evaluating combinations