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GSTA2X was expressed in Pichia pastoris X-33 and its enzymatic properties for catalyzing the conjugation of AFBO with GSH showed that the optimum temperature and pH were 2025 C and 7.68.6 as well as the enzymatic kinetic parameter V max was 0.23 nmol/min/mg and the Michaelis constant was 86.05 mol/L with the AFB 1 as substrate
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GSH is exclusively degraded at the extracellular level by cells expressing -glutamyltranspeptidase (GGT), such as those of the hepatobiliary tree and of other organs like the heart, kidney, lungs, pancreas, and seminal vesicles
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