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Letter of Medical Necessity (LMN) from the prescribing clinician for weight-loss use, stating the diagnosis, the recommended treatment, the duration, and why the medication is medically necessary

Pellets were then resuspended in FACS buffer and incubated with Cd45-FITC (rat IgG2b), Cd45-Brillant Violet 570 (rat IgG2b,), Cd11b-APC (rat IgG2b,), Cd11b-(Mac1)-PERCy5.5 (rat IgG2b,), F4/80-PECy7 (rat IgG2a,), Ly6g-APC-Cy7 (rat IgG2a,), Cd11c-PE (hamster IgG), Cd11c-APC (hamster IgG), Cd206-FITC (rat IgG2a,), Cd3-PECy7 (hamster IgG), SiglecF-PCy7 (rat IgG2a,), NK1.1-APC (mouse IgG2a,), Cd8-Brilliant Violet 421 (rat IgG2a,), Cd4-PERCy5.5 (rat IgG2b,), TCR-FITC (hamster IgG) and IL4-PE (rat IgG1) antibodies (Supplementary Data 1) or their corresponding isotype controls for 20 min at RT

At baseline, the patients had no statistically significant differences except for more patients in the case group having a diagnosis of diabetes

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K.PaskettE