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Immunohistochemistry was carried out using an automated immunohistochemical stainer according to the manufacturers guidelines (Bond-Max autostainer, Leica, Wetzlar, Germany), after dewaxing and rehydrating paraffin sections and antigen retrieval by pretreatment with high temperature at pH 9

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miRNA-23b, miR-221/222 levels positively correlate with the ESR, rheumatoid factor (RF), CRP, disease activity score (DAS), and anti-citrullinated protein antibodies (ACPA), which will become promising targets for RA detection (31, 204)

At an OD 600 of 0.50.6, expression was induced with 0.1 mM IPTG and the substrate TML was added at a concentration of 1 mM

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