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Phytother Res 34:214226 Aoyama S, Kase H, Borrelli E (2000) Rescue of locomotor impairment in dopamine D2 receptor-deficient mice by an adenosine A2A receptor antagonist

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The gradients were applied starting with 335% Solvent B over 90 min, then 2550% solvent B over 20 min, 5095% solvent B over 5 min, and a 100% solvent B for 10 min, and then 3% solvent B for 10 min