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As a critical regulator of ferroptosis, GPX4 is the most potent anti-lipid peroxidase in the cell (29, 30)

After incubation with the primary antibody, the cells were washed with PBST and stained with secondary antibodies (Goat Anti-Rabbit IgG H&L Alexa Fluor 488, Abcam, ab150077

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Common research applications include single-receptor pharmacology (semaglutide and liraglutide as GLP-1 reference agonists), dual-receptor comparison studies (tirzepatide for GIP/GLP-1, mazdutide and survodutide for GLP-1/glucagon), triple-receptor research (retatrutide), and multi-pathway combinations using amylin agonists alongside incretin agonists (cagri-sema, retatrutide+cagrilintide)