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Bacterial cells were collected by centrifugation at 4,000 g for 20 min at 4 C and the cell pellet was resuspended in buffer (50 mM NaH 2 PO 4 and 300 mM NaCl, pH 6.0)

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This reduces condensation in the vial when the seal is broken

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This prevents the chemical reactions, like hydrolysis, that occur in a liquid environment and dramatically slows down any potential degradation.