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If cysteine modification is a component required for either HLA-DRB1 binding or TCR recognition, then omission may alter results and potentially fail to highlight immunogenicity risk Presentation of peptides from biotherapeutic proteins is a necessary, but not sufficient, step for developing an ADA response

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Holm SK, Johansen VBI, Clemmensen C

Ltd, inserting the DSIP-CBBBP gene sequence between the XhoI and XbaI sites

Limitations: The interaction between the four peptides in a single blend has not been formally studied from a toxicological standpoint