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Cell cultures were harvested after 3 days and washed with ice-cold 1 PBS

Severe infiltration by inflammatory cells, synovial hyperplasia and bone/cartilage erosion were observed in the control and 100 mg/kg/day acarbose-treated mice

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In one study, pressure inside a spinal disc dropped to below 100 mm Hg when VAX-D was applied, showing that the treatment can effectively decompress the discs (Ramos et al., 1994)

For chronic conditions or maintenance, 8-12 week protocols are common