and AlexaFluor 594 donkey anti-mouse antibody, Jackson ImmunoResearch 711-605-152, 1:1,000) in blocking buffer for 1 h at room temperature, washed twice with blocking buffer, stained with 0.5 g ml 1 DAPI in PBS (Invitrogen, D1306), and washed three times with PBS
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of India) for NET-SRF and GRF respectively