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Complementary DNA (cDNA) was synthesized using the Evo M-MLV RT Premix for qPCR

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Previous clinical studies demonstrated that ICAM-1 expression is significantly elevated in the vitreous of patients with early NPDR, correlating with increased retinal vascular permeability ( 2.2.2 Inflammasome The inflammasome particularly the NLRP3, has emerged as a key contributor to retinal inflammation in early DR