Altered expression of Alzheimers disease-related genes in the cerebellum of autistic patients: a model for disrupted brain connectome and therapy
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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)
The researchers observed a significant increase in glutathione (GSH) synthesis (approximately 85 percent) and a 64 percent rise in total GSH levels in red blood cells
A GFP driven by its own promoter is also present in the construct to allow visualization of viral spreading in the brain structure upon intracranial injection
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