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In that same study, pre-treated cells with 3-methyladenine (autophagy inhibitor) demonstrated a decrease in the apoptotic process, thus showing that autophagy can affect the onset of apoptosis (74)

Briefly, the HL-1 cardiomyocytes were incubated with serum-free media containing JC-1 (10 M) for 30 min (37 C, in dark)

Concentration and dose are not the same

While some individuals may notice a subtle shift in mental clarity or energy within the first few days, for most, the real work happens at a cellular level over weeks and months

doi:10.1016/j.survophthal.2023.07.002 108