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Heparin plasma was incubated with chromogen (0.37 M N,N-Diethyl-1,4phenylendiammomiumsulphate, Merck, Darmstadt, Germany), FeSO 4 (6 mM, VWR International, Radnor, PA, USA) and acetate buffer (0.01 M, pH 5.0, AppliChem, Darmstadt, Germany) for 120 min at 37C

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In children, appropriate antibiotic treatment may decrease the risk of complications such as rheumatic fever

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