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In addition, age dependent accretion of dysfunctional mitochondria elevates stress level, which is exhibited by high levels oxidized products such as protein carbonyls and lipid peroxides 42

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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)