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Several loading methods are available for modifying exosomes, including incubation, sonication, electroporation, transfection, extrusion, freeze-thaw cycles, thermal shock, saponin-assisted loading, pH gradient methods, and hypotonic dialysis

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Researchers who have struggled with tirzepatide losing effectiveness over time might consider whether switching to CagriSema when it becomes available could reignite weight loss through the different amylin-based mechanism

Front Endocrinol 2021;12:626390

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