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Cells were washed and incubated in KRB buffer (118.5 mM NaCl, 4.8 mM KCl, 2.7 mM CaCl 2 , 1.2 mM KH 2 PO 4 , 1.1 mM MgSO 4 7H 2 O, 25 mM NaHCO 3 , and 4% bovine serum albumin, pH 7.4) and treated with low (2.8 mM) or high (16.7 mM) glucose for 2 hours at 37