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CS-PE cells were cultured in CSC Complete Recombinant Medium (Cell Systems Corporation, Kirkland, WA, USA), and HPDE cells were cultured in HuMedia-KG2 medium (Kurabo, Osaka, Japan)

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Additionally, natural remedies like psyllium husk or flaxseed can serve as gentle alternatives to synthetic laxatives, providing fiber while promoting healthy bowel function

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