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Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using a QuantiTect SYBR-Green RT-PCR kit (Qiagen, Venlo, The Netherlands) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA)

GLP-1 receptor agonists have demonstrated consistent reductions in major adverse cardiovascular events in large cardiovascular outcome trials involving patients with type 2 diabetes and established atherosclerotic cardiovascular disease

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Front Mol Biosci 2021 Oct 7;8:698698

These findings underscore the potential for peptide sequence optimization to fine-tune receptor selectivity or balanced activation, providing tailored approaches for treating different diseases 14

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