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DOSAGE GUIDE AND PREPARATION: Doses in relation to body weight are: Estimating Potential for Hepatotoxicity The following nomogram has been developed to estimate the probability that plasma levels in relation to intervals post ingestion will result in hepatotoxicity

Assessment of mitochondrial function Ten numbers of anesthetized twenty day old adult flies were dissected in cold Drosophila Schneiders Medium (DSM)

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)

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De Bruyne, E