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Insert the needle at a 45 to 90 degree angle (90 degrees for most people with adequate subcutaneous tissue)

Front Endocrinol (2022) 13:1030151

Cell culture and treatments The intestinal endocrine L cell line STC-1 and islet cell line NIT-1 were cultured in RPMI 1640 medium supplemented with 5 mM glucose, 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin in 100 mm cell culture dishes at 37 C in a 5% CO 2 atmosphere

While originally approved to help manage type 2 diabetes, it is also prescribed off-label by healthcare providers as part of medical weight loss programs

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