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Tap water contains chlorine, fluoride, minerals, and bacteria that degrade peptides and introduce contamination

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Dosing is extrapolated from preclinical models [1] [6]

To address this gap, microRNA gain and loss-of-function studies could be performed in specific islet cell populations using immortalized cell lines or whole islets or with human pluripotent stem cells-derived islets from T1D patients to account for genetic susceptibility

Retention times, full scans, and MS/MS spectra were compared with those obtained from the analysis of reference standards or suitable databases such as PubChem or MassBank, which are public repositories of mass spectra