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Once all the water is in the vial, remove the syringe

In parental (wild-type mtDNA) cells, 5FU drives significant increases in mitochondrial mass

Then 80% of the supernatant was taken and dried down before reconstitution in 100 l of mobile phase A (90:5:5 ACN:H 2 O:100 mM ammonium formate (AmFm) in H 2 O) and vortexed for ~10 s

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However, given the lack of proven benefits and documented risks, medical professionals advise all populations to avoid the practice