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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)

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Induction of monophasic EAE To induce monophasic EAE, 8/15-week-old male littermate mice were immunized by subcutaneous injection with an emulsion of 200 g myelin oligodendrocyte glycoprotein (MOG 3555 ) peptide in 100 l sterile phosphate buffered solution (PBS) and an equal volume of complete Freunds adjuvant (CFA), supplemented with M